› 5yo4

X-ray diffraction
1.37Å resolution

Crystal Structure of B562RIL with engineered disulfide bond K27C-A79C

Released:
DOI: 10.2210/pdb5yo4/pdb
PDB entry 5yo4 coloured by chain, front view.
PDB entry 5yo4 coloured by chain, side view.
PDB entry 5yo4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Protein crystal quality oriented disulfide bond engineering.
Pu M, Xu Z, Peng Y, Hou Y, Liu D, Wang Y, Liu H, Song G, Liu ZJ
Protein Cell 9 659-663 (2018)
PMID: 29039033

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2221
Unit cell:
a: 41.766Å b: 51.287Å c: 89.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.212
Expression system: Escherichia coli

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Citations

7 citation in other articles

Full-length human GLP-1 receptor structure without orthosteric ligands.
Wu et al. (2020)
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