› 5ypy

X-ray diffraction
1.97Å resolution

Crystal structure of IlvN. Val-1c

Released:
DOI: 10.2210/pdb5ypy/pdb
PDB entry 5ypy coloured by chain, front view.
PDB entry 5ypy coloured by chain, side view.
PDB entry 5ypy coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases.
Bansal A, Karanth NM, Demeler B, Schindelin H, Sarma SP
Biochemistry 58 1992-2008 (2019)
PMID: 30887800

Function and Biology Details

Reaction catalysed: 
2 pyruvate = 2-acetolactate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142354 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetolactate synthase isozyme 1 small subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 98 amino acids
Theoretical weight: 11.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADF8 (Residues: 1-96; Coverage: 100%)
Gene names: JW3645, b3670, ilvN
Sequence domains: ACT domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand VAL 4 x VAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 110.519Å b: 74.51Å c: 63.27Å
α: 90° β: 97.58° γ: 90°
R-values:
R R work R free
0.2 0.198 0.242
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases.
Bansal et al. (2019)