› 5yyn

X-ray diffraction
3Å resolution

Crystal structures of E.coli arginyl-trna synthetase (argrs) in complex with substrate TRNA(Arg)

Released:
DOI: 10.2210/pdb5yyn/pdb
PDB entry 5yyn coloured by chain, front view.
PDB entry 5yyn coloured by chain, side view.
PDB entry 5yyn coloured by chain, top view.
Source organisms:
Entry authors: Zhou M, Ye S, Stephen P, Zhang RG, Wang ED, Giege R, Lin SX

Function and Biology Details

Reaction catalysed: 
ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145965 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Arginine--tRNA ligase Chains: A, C
Molecule details ›
Chains: A, C
Length: 586 amino acids
Theoretical weight: 65.78 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P11875 (Residues: 1-577; Coverage: 100%)
Gene names: JW1865, argS, b1876
Sequence domains:
tRNA Chains: B, D
Molecule details ›
Chains: B, D
Length: 77 nucleotides
Theoretical weight: 24.82 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
Sequence domains: tRNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 102.241Å b: 102.241Å c: 480.567Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.22 0.268
Expression system: Escherichia coli

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