› 5z66

X-ray diffraction
1.8Å resolution

Structure of periplasmic trehalase from Diamondback moth gut bacteria complexed with validoxylamine

Released:
DOI: 10.2210/pdb5z66/pdb
PDB entry 5z66 coloured by chain, front view.
PDB entry 5z66 coloured by chain, side view.
PDB entry 5z66 coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Mechanistic insights into enzymatic catalysis by trehalase from the insect gut endosymbiont Enterobacter cloacae.
Adhav A, Harne S, Bhide A, Giri A, Gayathri P, Joshi R
FEBS J 286 1700-1716 (2019)
PMID: 30657252

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic trehalase Chain: A
Molecule details ›
Chain: A
Length: 563 amino acids
Theoretical weight: 63.17 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
Structure domains: Glycosyltransferase

Ligands and Environments

3 bound ligands:
Ligand SO4 8 x SO4
Ligand VDM 1 x VDM
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 122.03Å b: 51.45Å c: 96.4Å
α: 90° β: 106.95° γ: 90°
R-values:
R R work R free
0.164 0.163 0.194
Expression system: Escherichia coli

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Citations

1 review citation

Bacterial α-diglucoside metabolism: perspectives and potential for biotechnology and biomedicine.
Garcia et al. (2021)
6 other articles