› 5z6w

X-ray diffraction
3.2Å resolution

Crystal structure of paFAN1 bound to 2nt 5'flap DNA with gap with Manganese

Released:
DOI: 10.2210/pdb5z6w/pdb
PDB entry 5z6w coloured by chain, front view.
PDB entry 5z6w coloured by chain, side view.
PDB entry 5z6w coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanism of DNA interstrand cross-link unhooking by the bacterial FAN1 nuclease.
Jin H, Roy U, Lee G, Schärer OD, Cho Y
J Biol Chem 293 6482-6496 (2018)
PMID: 29514982

Function and Biology Details

Reaction catalysed: 
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119483 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Fanconi-associated nuclease 1 homolog Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.91 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I2N0 (Residues: 1-559; Coverage: 100%)
Gene names: PA1865, fan1
Sequence domains:
DNA (5'-D(P*GP*TP*TP*GP*GP*GP*AP*TP*TP*G)-3') Chain: H
Molecule details ›
Chain: H
Length: 10 nucleotides
Theoretical weight: 3.13 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*AP*TP*TP*CP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.89 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*AP*AP*TP*GP*TP*GP*TP*CP*TP*CP*AP*AP*TP*CP*CP*CP*AP*AP*CP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.32 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P212121
Unit cell:
a: 80.874Å b: 104.969Å c: 105.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.238
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

FAN1's protection against CGG repeat expansion requires its nuclease activity and is FANCD2-independent.
Zhao et al. (2021)

1 mention without citation

Structural mechanism of DNA interstrand cross-link unhooking by the bacterial FAN1 nuclease.
Jin et al. (2018)