› 5zgn

X-ray diffraction
2.24Å resolution

The crystal structure of KacTA-DNA complex

Released:
DOI: 10.2210/pdb5zgn/pdb
PDB entry 5zgn coloured by chain, front view.
PDB entry 5zgn coloured by chain, side view.
PDB entry 5zgn coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae HS11286
Entry authors: Qian HL, Yao QQ, Gan JH, Ou HY

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101873 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DUF1778 domain-containing protein Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 88 amino acids
Theoretical weight: 10.15 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae HS11286
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: A0A0H3GLZ1 (Residues: 1-88; Coverage: 100%)
Gene name: KPHS_05880
Sequence domains: Protein of unknown function (DUF1778)
N-acetyltransferase domain-containing protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 177 amino acids
Theoretical weight: 20.09 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae HS11286
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: A0A0H3GMP0 (Residues: 1-177; Coverage: 100%)
Gene name: KPHS_05890
Sequence domains: Acetyltransferase (GNAT) domain
DNA (27-MER) Chain: G
Molecule details ›
Chain: G
Length: 27 nucleotides
Theoretical weight: 8.32 KDa
DNA (27-MER) Chain: H
Molecule details ›
Chain: H
Length: 27 nucleotides
Theoretical weight: 8.27 KDa

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 57.046Å b: 62.044Å c: 61.994Å
α: 93.83° β: 93.99° γ: 93.93°
R-values:
R R work R free
0.216 0.214 0.255
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T

Quick links