› 5zi6

X-ray diffraction
2.2Å resolution

The RING domain structure of MEX-3C

Released:
DOI: 10.2210/pdb5zi6/pdb
PDB entry 5zi6 coloured by chain, front view.
PDB entry 5zi6 coloured by chain, side view.
PDB entry 5zi6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional characterization of hMEX-3C Ring finger domain as an E3 ubiquitin ligase.
Moududee SA, Jiang Y, Gilbert N, Xie G, Xu Z, Wu J, Gong Q, Tang Y, Shi Y
Protein Sci 27 1661-1669 (2018)
PMID: 30095198

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding E3 ubiquitin-protein ligase MEX3C Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 55 amino acids
Theoretical weight: 6.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q5U5Q3 (Residues: 605-659; Coverage: 8%)
Gene names: BM-013, MEX3C, RKHD2, RNF194
Sequence domains: Zinc finger, C3HC4 type (RING finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 16 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 37.135Å b: 44.567Å c: 67.157Å
α: 92.43° β: 90.5° γ: 91.39°
R-values:
R R work R free
0.209 0.207 0.243
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

RNA-Binding Proteins as Regulators of Migration, Invasion and Metastasis in Oral Squamous Cell Carcinoma.
Weiße et al. (2020)
1 more

2 mentions without citation

Oncogenic Potential of the Dual-Function Protein MEX3A.
Lederer et al. (2021)
1 more