› 5zro

X-ray diffraction
1.41Å resolution

M. smegmatis antimutator protein MutT2 in complex with 5mdCTP

Released:
DOI: 10.2210/pdb5zro/pdb
PDB entry 5zro coloured by chain, front view.
PDB entry 5zro coloured by chain, side view.
PDB entry 5zro coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural insights into the specificity and catalytic mechanism of mycobacterial nucleotide pool sanitizing enzyme MutT2.
Singh A, Mohammad Arif S, Biak Sang P, Varshney U, Vijayan M
J Struct Biol 204 449-456 (2018)
PMID: 30312643

Function and Biology Details

Reaction catalysed: 
8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-oxo-dGTP diphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.08 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0R2K6 (Residues: 1-130; Coverage: 100%)
Gene name: MSMEG_5148
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

2 bound ligands:
Ligand 523 2 x 523
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 31.61Å b: 72.76Å c: 57.86Å
α: 90° β: 104.7° γ: 90°
R-values:
R R work R free
0.143 0.141 0.184
Expression system: Escherichia coli

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Citations

2 mentions without citation

Superior Radiation Resistance of ZrO2-Modified W Composites.
Cui et al. (2022)
1 more