› 5zw2

X-ray diffraction
1.8Å resolution

FAD complex of PigA

Released:
DOI: 10.2210/pdb5zw2/pdb
PDB entry 5zw2 coloured by chain, front view.
PDB entry 5zw2 coloured by chain, side view.
PDB entry 5zw2 coloured by chain, top view.
Source organism: Serratia sp. ATCC 39006
Primary publication:
Crystal Structure of PigA: A Prolyl Thioester-Oxidizing Enzyme in Prodigiosin Biosynthesis.
Lee CC, Ko TP, Chen CT, Chan YT, Lo SY, Chang JY, Chen YW, Chung TF, Hsieh HJ, Hsiao CD, Wang AH
Chembiochem 20 193-202 (2019)
PMID: 30095206

Function and Biology Details

Reaction catalysed: 
L-prolyl-[peptidyl-carrier protein] + 2 electron-transfer flavoprotein = 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] + 2 reduced electron-transfer flavoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-prolyl-[peptidyl-carrier protein] dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 402 amino acids
Theoretical weight: 43.95 KDa
Source organism: Serratia sp. ATCC 39006
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5W271 (Residues: 1-386; Coverage: 100%)
Gene name: pigA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand FAD 1 x FAD
Ligand O4B 2 x O4B
Ligand EDO 7 x EDO
Ligand ACT 2 x ACT
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: I222
Unit cell:
a: 85.014Å b: 85.82Å c: 145.744Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.174
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Type II non-ribosomal peptide synthetase proteins: structure, mechanism, and protein-protein interactions.
Jaremko et al. (2020)