› 6a43

X-ray diffraction
2.4Å resolution

R1EN(5-225)-ubiquitin fusion

Released:
DOI: 10.2210/pdb6a43/pdb
PDB entry 6a43 coloured by chain, front view.
PDB entry 6a43 coloured by chain, side view.
PDB entry 6a43 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal Structure Determination of Ubiquitin by Fusion to a Protein That Forms a Highly Porous Crystal Lattice.
Maita N
J Am Chem Soc 140 13546-13549 (2018)
PMID: 30299944

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse transcriptase domain-containing protein; Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.35 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7M4J4 (Residues: 5-225; Coverage: 21%)
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P321
Unit cell:
a: 141.46Å b: 141.46Å c: 37.993Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
4 other articles