› 6alz

X-ray diffraction
2.21Å resolution

Crystal structure of Protein Phosphatase 1 bound to the natural inhibitor Tautomycetin

Released:
DOI: 10.2210/pdb6alz/pdb
PDB entry 6alz coloured by chain, front view.
PDB entry 6alz coloured by chain, side view.
PDB entry 6alz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
PP1:Tautomycetin Complex Reveals a Path toward the Development of PP1-Specific Inhibitors.
Choy MS, Swingle M, D'Arcy B, Abney K, Rusin SF, Kettenbach AN, Page R, Honkanen RE, Peti W
J Am Chem Soc 139 17703-17706 (2017)
PMID: 29156132

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-158547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 34.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62136 (Residues: 7-300; Coverage: 89%)
Gene names: PPP1A, PPP1CA
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

4 bound ligands:
Ligand MN 4 x MN
Ligand BKM 2 x BKM
Ligand DMS 1 x DMS
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 66.199Å b: 78.855Å c: 133.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.216
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein phosphatases in the regulation of mitosis.
Nilsson J. (2019)
3 more

2 mentions without citation

New targets for HIV drug discovery.
Puhl et al. (2019)
1 more