› 6apl

X-ray diffraction
2.35Å resolution

Crystal Structure of human ST6GALNAC2 in complex with CMP

Released:
DOI: 10.2210/pdb6apl/pdb
PDB entry 6apl coloured by chain, front view.
PDB entry 6apl coloured by chain, side view.
PDB entry 6apl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Expression system for structural and functional studies of human glycosylation enzymes.
Moremen KW, Ramiah A, Stuart M, Steel J, Meng L, Forouhar F, Moniz HA, Gahlay G, Gao Z, Chapla D, Wang S, Yang JY, Prabhakar PK, Johnson R, Rosa MD, Geisler C, Nairn AV, Seetharaman J, Wu SC, Tong L, Gilbert HJ, LaBaer J, Jarvis DL
Nat Chem Biol 14 156-162 (2018)
PMID: 29251719

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 374 amino acids
Theoretical weight: 42.27 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UJ37 (Residues: 1-374; Coverage: 100%)
Gene names: SIAT7B, SIATL1, ST6GALNAC2, STHM
Sequence domains: Glycosyltransferase family 29 (sialyltransferase)

Ligands and Environments

2 bound ligands:
Ligand C5P 6 x C5P
Ligand NAG 10 x NAG
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P1
Unit cell:
a: 71.168Å b: 71.125Å c: 138.608Å
α: 103.69° β: 97.26° γ: 103.03°
R-values:
R R work R free
0.2 0.196 0.236
Expression system: Homo sapiens

Quick links

Citations

18 review citations

Insights into the role of sialylation in cancer progression and metastasis.
Dobie et al. (2021)
17 more

6 mentions without citation

Protein and Glycan Mimicry in HIV Vaccine Design.
Seabright et al. (2019)
5 more