› 6apx

X-ray diffraction
2.49Å resolution

Crystal structure of human dual specificity phosphatase 1 catalytic domain (C258S) as a maltose binding protein fusion in complex with the monobody YSX1

Released:
DOI: 10.2210/pdb6apx/pdb
PDB entry 6apx coloured by chain, front view.
PDB entry 6apx coloured by chain, side view.
PDB entry 6apx coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the human dual specificity phosphatase 1 catalytic domain.
Gumpena R, Lountos GT, Raran-Kurussi S, Tropea JE, Cherry S, Waugh DS
Protein Sci 27 561-567 (2018)
PMID: 29052270

Function and Biology Details

Reactions catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142528 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dual specificity protein phosphatase 1; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 520 amino acids
Theoretical weight: 57.1 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P28562 (Residues: 172-314; Coverage: 39%)
Gene names: CL100, DUSP1, JW3994, MKP1, PTPN10, VH1, b4034, malE
Sequence domains:
Monobody YSX1 Chain: B
Molecule details ›
Chain: B
Length: 96 amino acids
Theoretical weight: 10.42 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 105.802Å b: 105.802Å c: 181.463Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Monobodies as enabling tools for structural and mechanistic biology.
Hantschel et al. (2020)
2 other articles

4 mentions without citation

Crystal structure of the human dual specificity phosphatase 1 catalytic domain.
Gumpena et al. (2018)
3 more