› 6aqb

X-ray diffraction
1.5Å resolution

Structure of the SH3 domain of MLK3 bound to peptide generated from phage display

Released:
DOI: 10.2210/pdb6aqb/pdb
PDB entry 6aqb coloured by chain, front view.
PDB entry 6aqb coloured by chain, side view.
PDB entry 6aqb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of two distinct peptide-binding pockets in the SH3 domain of human mixed-lineage kinase 3.
Kokoszka ME, Kall SL, Khosla S, McGinnis JE, Lavie A, Kay BK
J Biol Chem 293 13553-13565 (2018)
PMID: 29980598

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172516 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase 11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 73 amino acids
Theoretical weight: 7.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16584 (Residues: 41-108; Coverage: 8%)
Gene names: MAP3K11, MLK3, PTK1, SPRK
Sequence domains: Variant SH3 domain

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 80.51Å b: 45.74Å c: 49.66Å
α: 90° β: 120.88° γ: 90°
R-values:
R R work R free
0.189 0.187 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Phage display derived peptides for Alzheimer's disease therapy and diagnosis.
Zhang et al. (2022)
1 more

1 mention without citation

Identification of two distinct peptide-binding pockets in the SH3 domain of human mixed-lineage kinase 3.
Kokoszka et al. (2018)