› 6aru

X-ray diffraction
3.2Å resolution

Structure of Cetuximab Fab mutant in complex with EGFR extracellular domain

Released:
DOI: 10.2210/pdb6aru/pdb
PDB entry 6aru coloured by chain, front view.
PDB entry 6aru coloured by chain, side view.
PDB entry 6aru coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Christie M, Christ D

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-132675 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 622 amino acids
Theoretical weight: 69.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00533 (Residues: 25-640; Coverage: 52%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains:
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 23.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8TCD0 (Residues: 21-239; Coverage: 98%)
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin gamma-1 heavy chain Chain: C
Molecule details ›
Chain: C
Length: 222 amino acids
Theoretical weight: 23.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOX5 (Residues: 1-105, 106-222; Coverage: 49%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 77.495Å b: 70.444Å c: 149.079Å
α: 90° β: 100.95° γ: 90°
R-values:
R R work R free
0.234 0.232 0.267
Expression system: Homo sapiens

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