› 6asa

X-ray diffraction
2.54Å resolution

KRAS mutant-D33E in GDP-bound

Released:
DOI: 10.2210/pdb6asa/pdb
PDB entry 6asa coloured by chain, front view.
PDB entry 6asa coloured by chain, side view.
PDB entry 6asa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
KRAS Switch Mutants D33E and A59G Crystallize in the State 1 Conformation.
Lu J, Bera AK, Gondi S, Westover KD
Biochemistry 57 324-333 (2018)
PMID: 29235861

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-167; Coverage: 88%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 34.527Å b: 47.075Å c: 90.765Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.186 0.264
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The current understanding of KRAS protein structure and dynamics.
Pantsar T. (2020)
2 more

2 mentions without citation

KRAS Switch Mutants D33E and A59G Crystallize in the State 1 Conformation.
Lu et al. (2018)
1 more