› 6at0

X-ray diffraction
1.28Å resolution

Chromodomain HP1 with a p-nitro-L-phenylalanine mutation at position 24 bound to histone H3 peptide containing trimethyl lysine

Released:
DOI: 10.2210/pdb6at0/pdb
PDB entry 6at0 coloured by chain, front view.
PDB entry 6at0 coloured by chain, side view.
PDB entry 6at0 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Investigation of Trimethyllysine Binding by the HP1 Chromodomain via Unnatural Amino Acid Mutagenesis.
Baril SA, Koenig AL, Krone MW, Albanese KI, He CQ, Lee GY, Houk KN, Waters ML, Brustad EM
J Am Chem Soc 139 17253-17256 (2017)
PMID: 29111699

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135450 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heterochromatin protein 1 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 8.62 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05205 (Residues: 17-76; Coverage: 29%)
Gene names: CG8409, HP1, HP1a, Su(var)2-5, Su(var)205
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Histone H3 Chain: P
Molecule details ›
Chain: P
Length: 6 amino acids
Theoretical weight: 734 Da
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: P02299 (Residues: 6-11; Coverage: 4%)
Gene names: CG31613, CG33803, CG33806, CG33809, CG33812, CG33815, CG33818, CG33821, CG33824, CG33827, CG33830, CG33833, CG33836, CG33839, CG33842, CG33845, CG33848, CG33851, CG33854, CG33857, CG33860, CG33863, CG33866, His3, His3:CG31613, His3:CG33803, His3:CG33806, His3:CG33809, His3:CG33812, His3:CG33815, His3:CG33818, His3:CG33821, His3:CG33824, His3:CG33827, His3:CG33830, His3:CG33833, His3:CG33836, His3:CG33839, His3:CG33842, His3:CG33845, His3:CG33848, His3:CG33851, His3:CG33854, His3:CG33857, His3:CG33860, His3:CG33863, His3:CG33866

Ligands and Environments

No bound ligands
2 modified residues:
Ligand PPN 1 x PPN
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 34.417Å b: 76.857Å c: 76.479Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Trimethyllysine: From Carnitine Biosynthesis to Epigenetics.
Maas et al. (2020)
13 other articles

1 mention without citation

Investigation of Trimethyllysine Binding by the HP1 Chromodomain via Unnatural Amino Acid Mutagenesis.
Baril et al. (2017)