› 6atd

X-ray diffraction
2.5Å resolution

Oxidized SHP2 forms a disulfide bond between Cys367 and Cys459

Released:
DOI: 10.2210/pdb6atd/pdb
PDB entry 6atd coloured by chain, front view.
PDB entry 6atd coloured by chain, side view.
PDB entry 6atd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Redox Regulation of a Gain-of-Function Mutation (N308D) in SHP2 Noonan Syndrome.
Machado LESF, Critton DA, Page R, Peti W
ACS Omega 2 8313-8318 (2017)
PMID: 29214238

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 528 amino acids
Theoretical weight: 60.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06124 (Residues: 1-526; Coverage: 89%)
Gene names: PTP2C, PTPN11, SHPTP2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 46.292Å b: 216.135Å c: 55.709Å
α: 90° β: 97.25° γ: 90°
R-values:
R R work R free
0.197 0.195 0.245
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Setting sail: Maneuvering SHP2 activity and its effects in cancer.
Welsh et al. (2023)
1 more

3 mentions without citation

A novel partially open state of SHP2 points to a "multiple gear" regulation mechanism.
Tao et al. (2021)
2 more