6avf

X-ray diffraction
2.03Å resolution

Crystal structure of the KFJ5 TCR-NY-ESO-1-HLA-B*07:02 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-100920 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Beta-2-microglobulin Chain: M
Molecule details ›
Chain: M
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T cell receptor alpha variable 4 Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B4J268 (Residues: 18-109; Coverage: 100%)
Gene name: TRAV4
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
T cell receptor beta variable 28 Chain: B
Molecule details ›
Chain: B
Length: 244 amino acids
Theoretical weight: 28.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5B6 (Residues: 19-114; Coverage: 100%)
Gene names: TCRBV3S1, TRBV28
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Cancer/testis antigen 1 Chain: P
Molecule details ›
Chain: P
Length: 13 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78358 (Residues: 60-72; Coverage: 7%)
Gene names: CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, LAGE2, LAGE2A, LAGE2B
HLA class I histocompatibility antigen, B alpha chain Chain: H
Molecule details ›
Chain: H
Length: 362 amino acids
Theoretical weight: 40.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01889 (Residues: 1-362; Coverage: 100%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 64.366Å b: 67.679Å c: 105.716Å
α: 90° β: 102.93° γ: 90°
R-values:
R R work R free
0.206 0.204 0.256
Expression systems:
  • Escherichia coli
  • Not provided