› 6avi

X-ray diffraction
2.29Å resolution

Crystal Structure of Human PPARgamma Ligand Binding Domain in Complex with GW9662 and Nonanoic acid

Released:
DOI: 10.2210/pdb6avi/pdb
PDB entry 6avi coloured by chain, front view.
PDB entry 6avi coloured by chain, side view.
PDB entry 6avi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cooperative cobinding of synthetic and natural ligands to the nuclear receptor PPARγ.
Shang J, Brust R, Mosure SA, Bass J, Munoz-Tello P, Lin H, Hughes TS, Tang M, Ge Q, Kamenekca TM, Kojetin DJ
Elife 7 (2018)
PMID: 30575522

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisome proliferator-activated receptor gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 31.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37231 (Residues: 231-505; Coverage: 55%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

2 bound ligands:
Ligand GW9 2 x GW9
Ligand KNA 3 x KNA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 92.572Å b: 61.743Å c: 118.376Å
α: 90° β: 102.15° γ: 90°
R-values:
R R work R free
0.253 0.249 0.314
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Fatty Acid Signaling Mechanisms in Neural Cells: Fatty Acid Receptors.
Falomir-Lockhart et al. (2019)
10 more

2 mentions without citation

Cooperative cobinding of synthetic and natural ligands to the nuclear receptor PPARγ.
Shang et al. (2018)
1 more