Function and Biology Details
Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Ras guanine-nucleotide exchange factors catalytic domain
- Small GTPase
- P-loop containing nucleoside triphosphate hydrolase
- Ras-like guanine nucleotide exchange factor, N-terminal
- Small GTPase, Ras-type
- Small GTP-binding protein domain
- Ras guanine-nucleotide exchange factor, catalytic domain superfamily
- Ras-like guanine nucleotide exchange factor
1 more domain
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133970 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RAS guanyl-releasing protein 4
Molecule details ›
Chains: A, C, E, G, I, K
Length: 418 amino acids
Theoretical weight: 46.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: RasGEF domain
Structure domains: Ras guanine-nucleotide exchange factors catalytic domain
Length: 418 amino acids
Theoretical weight: 46.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q8TDF6 (Residues: 46-460; Coverage: 62%)
Sequence domains: RasGEF domain
Structure domains: Ras guanine-nucleotide exchange factors catalytic domain
GTPase HRas, N-terminally processed
Molecule details ›
Chains: B, D, F, H, J, L
Length: 170 amino acids
Theoretical weight: 19.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Length: 170 amino acids
Theoretical weight: 19.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ALS BEAMLINE 8.2.1
Spacegroup:
P3212
Expression system: Escherichia coli
