› 6b0b

X-ray diffraction
3.28Å resolution

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112324 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (4 distinct):
DNA dC->dU-editing enzyme APOBEC-3H Chains: A, E
Molecule details ›
Chains: A, E
Length: 186 amino acids
Theoretical weight: 21.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NTF7 (Residues: 1-181; Coverage: 91%)
Gene name: APOBEC3H
Sequence domains: APOBEC3
Fluorescent protein Chains: D, H
Molecule details ›
Chains: D, H
Length: 255 amino acids
Theoretical weight: 28.83 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D1MPT3 (Residues: 1-232; Coverage: 98%)
Gene names: PAmCherry, PAmCherry1
Sequence domains: Green fluorescent protein
RNA (5'-R(*UP*AP*AP*AP*AP*AP*AP*A)-3') Chains: B, F
Molecule details ›
Chains: B, F
Length: 8 nucleotides
Theoretical weight: 2.57 KDa
RNA (5'-R(*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 9 nucleotides
Theoretical weight: 2.71 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3112
Unit cell:
a: 101.293Å b: 101.293Å c: 211.217Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.353 0.353 0.362
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The Role of APOBECs in Viral Replication.
Xu et al. (2020)
6 more

11 mentions without citation

Examination of the APOBEC3 Barrier to Cross Species Transmission of Primate Lentiviruses.
Gaba et al. (2021)
10 more