› 6b1i

X-ray diffraction
2.3Å resolution

Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant

Released:
DOI: 10.2210/pdb6b1i/pdb
PDB entry 6b1i coloured by chain, front view.
PDB entry 6b1i coloured by chain, side view.
PDB entry 6b1i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disrupted Hydrogen-Bond Network and Impaired ATPase Activity in an Hsc70 Cysteine Mutant.
O'Donnell JP, Marsh HM, Sondermann H, Sevier CS
Biochemistry 57 1073-1086 (2018)
PMID: 29300467

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock cognate 71 kDa protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 44.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11142 (Residues: 5-381; Coverage: 58%)
Gene names: HSC70, HSP73, HSPA10, HSPA8
Sequence domains: Hsp70 protein

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand PO4 2 x PO4
Ligand ADP 2 x ADP
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 73.83Å b: 79.196Å c: 76.252Å
α: 90° β: 101.45° γ: 90°
R-values:
R R work R free
0.194 0.191 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Mechanisms of sensing and response to proteotoxic stress.
Santiago et al. (2020)
7 other articles

2 mentions without citation

Disrupted Hydrogen-Bond Network and Impaired ATPase Activity in an Hsc70 Cysteine Mutant.
O'Donnell et al. (2018)
1 more