› 6b20

X-ray diffraction
2.34Å resolution

Crystal structure of a complex between G protein beta gamma dimer and an inhibitory Nanobody regulator

Released:
DOI: 10.2210/pdb6b20/pdb
PDB entry 6b20 coloured by chain, front view.
PDB entry 6b20 coloured by chain, side view.
PDB entry 6b20 coloured by chain, top view.
Source organisms:
Primary publication:
Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor.
Gulati S, Jin H, Masuho I, Orban T, Cai Y, Pardon E, Martemyanov KA, Kiser PD, Stewart PL, Ford CP, Steyaert J, Palczewski K
Nat Commun 9 1996 (2018)
PMID: 29777099

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-230752 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 37.21 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62871 (Residues: 3-340; Coverage: 99%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(T) subunit gamma-T1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 60 amino acids
Theoretical weight: 7.13 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02698 (Residues: 7-66; Coverage: 81%)
Gene name: GNGT1
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain
Nanobody against G protein beta gamma dimer Chains: E, F
Molecule details ›
Chains: E, F
Length: 114 amino acids
Theoretical weight: 12.26 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand UNX 11 x UNX
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 72.66Å b: 77.23Å c: 101.66Å
α: 90° β: 109.8° γ: 90°
R-values:
R R work R free
0.203 0.2 0.249
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Targeting G protein-coupled receptor signalling by blocking G proteins.
Campbell et al. (2018)
11 more

4 mentions without citation

Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor.
Gulati et al. (2018)
3 more