› 6b27

X-ray diffraction
1.73Å resolution

Crystal structure of human STAC2 Tandem SH3 Domains (296-411) in complex with a CaV1.1 II-III loop peptide

Released:
DOI: 10.2210/pdb6b27/pdb
PDB entry 6b27 coloured by chain, front view.
PDB entry 6b27 coloured by chain, side view.
PDB entry 6b27 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into binding of STAC proteins to voltage-gated calcium channels.
Wong King Yuen SM, Campiglio M, Tung CC, Flucher BE, Van Petegem F
Proc Natl Acad Sci U S A 114 E9520-E9528 (2017)
PMID: 29078335

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171811 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SH3 and cysteine-rich domain-containing protein 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 120 amino acids
Theoretical weight: 13.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6ZMT1 (Residues: 296-411; Coverage: 28%)
Gene name: STAC2
Sequence domains:
Structure domains: SH3 Domains
Voltage-dependent L-type calcium channel subunit alpha-1S Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 14 amino acids
Theoretical weight: 1.63 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13698 (Residues: 747-760; Coverage: 1%)
Gene names: CACH1, CACN1, CACNA1S, CACNL1A3

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 47.92Å b: 114.65Å c: 144.678Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.216
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Skeletal muscle CaV1.1 channelopathies.
Flucher BE. (2020)
6 more

5 mentions without citation

Excitation-contraction coupling in skeletal muscle: recent progress and unanswered questions.
Shishmarev D. (2020)
4 more