› 6b6u

X-ray diffraction
1.35Å resolution

Pyruvate Kinase M2 mutant - S437Y

Released:
DOI: 10.2210/pdb6b6u/pdb
PDB entry 6b6u coloured by chain, front view.
PDB entry 6b6u coloured by chain, side view.
PDB entry 6b6u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Investigation of a Dimeric Variant of Pyruvate Kinase Muscle Isoform 2.
Srivastava D, Razzaghi M, Henzl MT, Dey M
Biochemistry 56 6517-6520 (2017)
PMID: 29182273

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B
Molecule details ›
Chains: A, B
Length: 525 amino acids
Theoretical weight: 57.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14618 (Residues: 7-531; Coverage: 99%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

9 bound ligands:
Ligand MG 2 x MG
Ligand OXL 2 x OXL
Ligand K 2 x K
Ligand CL 2 x CL
Ligand SCN 6 x SCN
Ligand PEG 1 x PEG
Ligand B3P 2 x B3P
Ligand PG4 1 x PG4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2
Unit cell:
a: 110.35Å b: 93.61Å c: 109.36Å
α: 90° β: 95.64° γ: 90°
R-values:
R R work R free
0.131 0.13 0.149
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Targeting Pyruvate Kinase M2 Phosphorylation Reverses Aggressive Cancer Phenotypes.
Apostolidi et al. (2021)
3 more

5 mentions without citation

Structural basis for allosteric regulation of pyruvate kinase M2 by phosphorylation and acetylation.
Nandi et al. (2020)
4 more