› 6b70

Electron Microscopy
3.7Å resolution

Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain and insulin

Released:
DOI: 10.2210/pdb6b70/pdb
PDB entry 6b70 coloured by chain, front view.
PDB entry 6b70 coloured by chain, side view.
PDB entry 6b70 coloured by chain, top view.
Source organisms:
Primary publication:
Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme.
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
Elife 7 (2018)
PMID: 29596046
Related structures: EMD-7062

Function and Biology Details

Reaction catalysed: 
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-134219 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 966 amino acids
Theoretical weight: 111.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14735 (Residues: 46-1011; Coverage: 95%)
Gene name: IDE
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Immunoglobulin gamma-1 heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 218 amino acids
Theoretical weight: 23.06 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DOX5 (Residues: 1-49, 50-219; Coverage: 48%)
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6GMX0 (Residues: 23-233; Coverage: 99%)
Sequence domains:
Structure domains: Immunoglobulins
Insulin Chains: a, c
Molecule details ›
Chains: a, c
Length: 110 amino acids
Theoretical weight: 11.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 1-110; Coverage: 100%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-7062
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
6 more

4 mentions without citation

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme.
Zhang et al. (2018)
3 more