› 6ba5

X-ray diffraction
1.62Å resolution

Potent and Selective Antitumor Activity of a T-Cell Engaging Bispecific Antibody Targeting a Membrane-Proximal Epitope of ROR1

Released:
DOI: 10.2210/pdb6ba5/pdb
PDB entry 6ba5 coloured by chain, front view.
PDB entry 6ba5 coloured by chain, side view.
PDB entry 6ba5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Potent and selective antitumor activity of a T cell-engaging bispecific antibody targeting a membrane-proximal epitope of ROR1.
Qi J, Li X, Peng H, Cook EM, Dadashian EL, Wiestner A, Park H, Rader C
Proc Natl Acad Sci U S A 115 E5467-E5476 (2018)
PMID: 29844189

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211888 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Variable domain of Light Chain, antibody R11 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 114 amino acids
Theoretical weight: 11.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Variable domain Heavy chain, antibody R11 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 117 amino acids
Theoretical weight: 12.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Inactive tyrosine-protein kinase transmembrane receptor ROR1 Chains: M, N, O, P
Molecule details ›
Chains: M, N, O, P
Length: 82 amino acids
Theoretical weight: 9.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01973 (Residues: 311-391; Coverage: 9%)
Gene names: NTRKR1, ROR1
Sequence domains: Kringle domain
Structure domains: Plasminogen Kringle 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 58.02Å b: 73.9Å c: 84.12Å
α: 89.86° β: 71.3° γ: 84.49°
R-values:
R R work R free
0.194 0.194 0.214
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Bispecific T-Cell Redirection versus Chimeric Antigen Receptor (CAR)-T Cells as Approaches to Kill Cancer Cells.
Strohl et al. (2019)
9 more

3 mentions without citation

Potent and selective antitumor activity of a T cell-engaging bispecific antibody targeting a membrane-proximal epitope of ROR1.
Qi et al. (2018)
2 more