› 6ban

X-ray diffraction
1.95Å resolution

Potent and Selective Antitumor Activity of a T-Cell Engaging Bispecific Antibody Targeting a Membrane-Proximal Epitope of ROR1

Released:
DOI: 10.2210/pdb6ban/pdb
PDB entry 6ban coloured by chain, front view.
PDB entry 6ban coloured by chain, side view.
PDB entry 6ban coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Potent and selective antitumor activity of a T cell-engaging bispecific antibody targeting a membrane-proximal epitope of ROR1.
Qi J, Li X, Peng H, Cook EM, Dadashian EL, Wiestner A, Park H, Rader C
Proc Natl Acad Sci U S A 115 E5467-E5476 (2018)
PMID: 29844189

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211889 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Variable domain of R11 Light Chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 114 amino acids
Theoretical weight: 11.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Variable domain R11 Heavy chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 117 amino acids
Theoretical weight: 12.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Inactive tyrosine-protein kinase transmembrane receptor ROR1 Chains: M, N, O, P
Molecule details ›
Chains: M, N, O, P
Length: 82 amino acids
Theoretical weight: 9.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01973 (Residues: 311-391; Coverage: 9%)
Gene names: NTRKR1, ROR1
Sequence domains: Kringle domain
Structure domains: Plasminogen Kringle 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 86.79Å b: 84.36Å c: 102.74Å
α: 90° β: 113.88° γ: 90°
R-values:
R R work R free
0.162 0.16 0.196
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Biology drives the discovery of bispecific antibodies as innovative therapeutics.
Nie et al. (2020)
9 more

1 mention without citation

Crystal structure of the kringle domain of human receptor tyrosine kinase-like orphan receptor 1 (hROR1).
Guarino et al. (2022)