› 6bbn

X-ray diffraction
3.51Å resolution

Crystal structure of a curved tubulin complex induced by the kinesin-13 Kif2A

Released:
DOI: 10.2210/pdb6bbn/pdb
PDB entry 6bbn coloured by chain, front view.
PDB entry 6bbn coloured by chain, side view.
PDB entry 6bbn coloured by chain, top view.
Source organisms:
Primary publication:
Ternary complex of Kif2A-bound tandem tubulin heterodimers represents a kinesin-13-mediated microtubule depolymerization reaction intermediate.
Trofimova D, Paydar M, Zara A, Talje L, Kwok BH, Allingham JS
Nat Commun 9 2628 (2018)
PMID: 29980677

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-126119 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
DARPin Chain: P
Molecule details ›
Chain: P
Length: 168 amino acids
Theoretical weight: 17.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain
Kinesin-like protein KIF2A Chain: E
Molecule details ›
Chain: E
Length: 420 amino acids
Theoretical weight: 47.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00139 (Residues: 153-553; Coverage: 57%)
Gene names: KIF2, KIF2A, KNS2
Sequence domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 4 x MG
Ligand GDP 2 x GDP
Ligand PO4 1 x PO4
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 145.052Å b: 82.188Å c: 147.658Å
α: 90° β: 109.33° γ: 90°
R-values:
R R work R free
0.239 0.236 0.287
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Regulation of microtubule dynamics, mechanics and function through the growing tip.
Gudimchuk et al. (2021)
6 more

2 mentions without citation

Parts list for a microtubule depolymerising kinesin.
Friel et al. (2018)
1 more