› 6bd2

X-ray diffraction
2.9Å resolution

Complex of 14-3-3 theta with an IRSp53 peptide doubly-phosphorylated at T340 and S366

Released:
DOI: 10.2210/pdb6bd2/pdb
PDB entry 6bd2 coloured by chain, front view.
PDB entry 6bd2 coloured by chain, side view.
PDB entry 6bd2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of IRSp53 inhibition by 14-3-3.
Kast DJ, Dominguez R
Nat Commun 10 483 (2019)
PMID: 30696821

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150978 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein theta Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27348 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAQ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
BAR/IMD domain-containing adapter protein 2 Chain: C
Molecule details ›
Chain: C
Length: 38 amino acids
Theoretical weight: 4.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UQB8 (Residues: 335-372; Coverage: 7%)
Gene name: BAIAP2

Ligands and Environments

5 bound ligands:
Ligand PEG 1 x PEG
Ligand 1PE 1 x 1PE
Ligand PG4 1 x PG4
Ligand PGE 1 x PGE
Ligand EDO 1 x EDO
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 69.883Å b: 75.002Å c: 100.216Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.254
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Pathways to Parkinson's disease: a spotlight on 14-3-3 proteins.
Giusto et al. (2021)
2 more