› 6bfq

X-ray diffraction
2.6Å resolution

The mechanism of GM-CSF inhibition by human GM-CSF auto-antibodies

Released:
DOI: 10.2210/pdb6bfq/pdb
PDB entry 6bfq coloured by chain, front view.
PDB entry 6bfq coloured by chain, side view.
PDB entry 6bfq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The mechanism of GM-CSF inhibition by human GM-CSF auto-antibodies suggests novel therapeutic opportunities.
Dhagat U, Hercus TR, Broughton SE, Nero TL, Cheung Tung Shing KS, Barry EF, Thomson CA, Bryson S, Pai EF, McClure BJ, Schrader JW, Lopez AF, Parker MW
MAbs 10 1018-1029 (2018)
PMID: 29969365

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230781 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Heavy chain Chains: A, C, E, H
Molecule details ›
Chains: A, C, E, H
Length: 225 amino acids
Theoretical weight: 23.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab Light Chain Chains: B, D, F, L
Molecule details ›
Chains: B, D, F, L
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Granulocyte-macrophage colony-stimulating factor Chains: G, I, J, K
Molecule details ›
Chains: G, I, J, K
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04141 (Residues: 18-144; Coverage: 100%)
Gene names: CSF2, GMCSF
Sequence domains: Granulocyte-macrophage colony-stimulating factor
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 95.437Å b: 100.535Å c: 101.866Å
α: 91.17° β: 117.96° γ: 108.65°
R-values:
R R work R free
0.232 0.23 0.264
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Neutralizing GM-CSF autoantibodies in pulmonary alveolar proteinosis, cryptococcal meningitis and severe nocardiosis.
Salvator et al. (2022)
3 more

1 mention without citation

The mechanism of GM-CSF inhibition by human GM-CSF auto-antibodies suggests novel therapeutic opportunities.
Dhagat et al. (2018)