› 6bgt

X-ray diffraction
2.7Å resolution

Structure of Trastuzumab Fab mutant in complex with Her2 extracellular domain

Released:
DOI: 10.2210/pdb6bgt/pdb
PDB entry 6bgt coloured by chain, front view.
PDB entry 6bgt coloured by chain, side view.
PDB entry 6bgt coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Christie M, Christ D

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-138064 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Receptor tyrosine-protein kinase erbB-2 Chain: C
Molecule details ›
Chain: C
Length: 658 amino acids
Theoretical weight: 72.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P04626 (Residues: 1-652; Coverage: 51%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7Z3Y4 (Residues: 23-236; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin gamma-1 heavy chain Chain: B
Molecule details ›
Chain: B
Length: 231 amino acids
Theoretical weight: 24.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOX5 (Residues: 1-49, 50-107, 108-222; Coverage: 49%)
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 62.86Å b: 114.46Å c: 204.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.248
Expression system: Homo sapiens

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