› 6bkj

X-ray diffraction
3.2Å resolution

Crystal Structure of Human Calpain-3 Protease Core in Complex with Leupeptin

Released:
DOI: 10.2210/pdb6bkj/pdb
PDB entry 6bkj coloured by chain, front view.
PDB entry 6bkj coloured by chain, side view.
PDB entry 6bkj coloured by chain, top view.
Source organisms:
Primary publication:
Structures of human calpain-3 protease core with and without bound inhibitor reveal mechanisms of calpain activation.
Ye Q, Campbell RL, Davies PL
J Biol Chem 293 4056-4070 (2018)
PMID: 29382717

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase activity
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149063 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calpain-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 382 amino acids
Theoretical weight: 44.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20807 (Residues: 46-419; Coverage: 46%)
Gene names: CANP3, CANPL3, CAPN3, NCL1
Sequence domains: Calpain family cysteine protease
Structure domains: Cysteine proteinases
Leupeptin Chains: F, G, H, I
Molecule details ›
Chains: F, G, H, I
Length: 4 amino acids
Theoretical weight: 430 Da
Source organism: Streptomyces roseus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 54.93Å b: 106.69Å c: 234.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.281 0.277 0.353
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Molecular and cellular basis of genetically inherited skeletal muscle disorders.
Dowling et al. (2021)
4 more

3 mentions without citation

Structures of human calpain-3 protease core with and without bound inhibitor reveal mechanisms of calpain activation.
Ye et al. (2018)
2 more