6bnt

X-ray diffraction
3.2Å resolution

Crystal structure of AP2 mu1 adaptin C-terminal domain with IRS-1 peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-152952 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AP-2 complex subunit mu Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 35.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96CW1 (Residues: 160-435; Coverage: 63%)
Gene names: AP2M1, CLAPM1, KIAA0109
Sequence domains: Adaptor complexes medium subunit family
Structure domains: Mu homology domain, subdomain B
Insulin receptor substrate 1 Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35568 (Residues: 607-620; Coverage: 1%)
Gene name: IRS1

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P64
Unit cell:
a: 125.334Å b: 125.334Å c: 74.818Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli