6bnv

Electron Microscopy
4.6Å resolution

CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-158576 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Unconventional myosin-VI Chains: I, J, K, L, M, N
Molecule details ›
Chains: I, J, K, L, M, N
Length: 816 amino acids
Theoretical weight: 93.21 KDa
Source organism: Sus scrofa
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q29122 (Residues: 2-818; Coverage: 65%)
Gene name: MYO6
Sequence domains: Myosin head (motor domain)
Actin, alpha skeletal muscle Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 373 amino acids
Theoretical weight: 41.56 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-373; Coverage: 99%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Calmodulin Chains: O, P, Q, R, S, T
Molecule details ›
Chains: O, P, Q, R, S, T
Length: 145 amino acids
Theoretical weight: 16.41 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62149 (Residues: 4-148; Coverage: 97%)
Gene names: CALM, CAM, RCJMB04_24e7
Sequence domains: EF-hand domain pair

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-7116
Expression system: Spodoptera frugiperda