› 6bsx

X-ray diffraction
1.65Å resolution

CRYSTAL STRUCTURE OF RHEB IN COMPLEX WITH COMPOUND 1 AT 1.65A RESOLUTION

Released:
DOI: 10.2210/pdb6bsx/pdb
PDB entry 6bsx coloured by chain, front view.
PDB entry 6bsx coloured by chain, side view.
PDB entry 6bsx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A small molecule inhibitor of Rheb selectively targets mTORC1 signaling.
Mahoney SJ, Narayan S, Molz L, Berstler LA, Kang SA, Vlasuk GP, Saiah E
Nat Commun 9 548 (2018)
PMID: 29416044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein Rheb Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 178 amino acids
Theoretical weight: 20.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15382 (Residues: 1-169; Coverage: 92%)
Gene names: RHEB, RHEB2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

5 bound ligands:
Ligand MG 4 x MG
Ligand ACT 4 x ACT
Ligand GDP 4 x GDP
Ligand E7S 4 x E7S
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 49.37Å b: 49.72Å c: 70.97Å
α: 89.99° β: 77.7° γ: 89.87°
R-values:
R R work R free
0.199 0.197 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Molecular mechanisms of dietary restriction promoting health and longevity.
Green et al. (2022)
19 more

2 mentions without citation

A small molecule inhibitor of Rheb selectively targets mTORC1 signaling.
Mahoney et al. (2018)
1 more