6bvb

X-ray diffraction
2Å resolution

Crystal structure of HIF-2alpha-pVHL-elongin B-elongin C

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-154088 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
von Hippel-Lindau disease tumor suppressor Chain: V
Molecule details ›
Chain: V
Length: 162 amino acids
Theoretical weight: 18.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40337 (Residues: 54-213; Coverage: 75%)
Gene name: VHL
Sequence domains:
Structure domains:
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
Endothelial PAS domain-containing protein 1 Chain: H
Molecule details ›
Chain: H
Length: 19 amino acids
Theoretical weight: 2.2 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q99814 (Residues: 523-540; Coverage: 2%)
Gene names: BHLHE73, EPAS1, HIF2A, MOP2, PASD2
Sequence domains: Hypoxia-inducible factor-1

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P43212
Unit cell:
a: 59.77Å b: 59.77Å c: 246.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.236
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided