› 6bwy

X-ray diffraction
2.9Å resolution

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119637 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA dC->dU-editing enzyme APOBEC-3G; Protection of telomeres protein 1 Chains: A, B, E, G
Molecule details ›
Chains: A, B, E, G
Length: 361 amino acids
Theoretical weight: 41.97 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: O13988 (Residues: 5-174; Coverage: 31%)
  • Canonical: Q9HC16 (Residues: 195-384; Coverage: 50%)
Gene names: APOBEC3G, MDS019, SPAC26H5.06, pot1
Sequence domains:
DNA (30-MER) Chains: C, D, F, I
Molecule details ›
Chains: C, D, F, I
Length: 30 nucleotides
Theoretical weight: 9.36 KDa

Ligands and Environments

2 bound ligands:
Ligand PO4 15 x PO4
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43
Unit cell:
a: 79.072Å b: 79.072Å c: 266.411Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.287
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural Insights into APOBEC3-Mediated Lentiviral Restriction.
Delviks-Frankenberry et al. (2020)
4 more

2 mentions without citation

Insights into DNA substrate selection by APOBEC3G from structural, biochemical, and functional studies.
Ziegler et al. (2018)
1 more