› 6bx6

X-ray diffraction
2.9Å resolution

AMP-Activated protein kinase (AMPK) inhibition by SBI-0206965: alpha 2 kinase domain bound to SBI-0206965

Released:
DOI: 10.2210/pdb6bx6/pdb
PDB entry 6bx6 coloured by chain, front view.
PDB entry 6bx6 coloured by chain, side view.
PDB entry 6bx6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
AMP-activated protein kinase selectively inhibited by the type II inhibitor SBI-0206965.
Dite TA, Langendorf CG, Hoque A, Galic S, Rebello RJ, Ovens AJ, Lindqvist LM, Ngoei KRW, Ling NXY, Furic L, Kemp BE, Scott JW, Oakhill JS
J Biol Chem 293 8874-8885 (2018)
PMID: 29695504

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-AMP-activated protein kinase catalytic subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P54646 (Residues: 6-279; Coverage: 50%)
Gene names: AMPK, AMPK2, PRKAA2
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand EDJ 1 x EDJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 38.43Å b: 54.783Å c: 143.188Å
α: 90° β: 96.25° γ: 90°
R-values:
R R work R free
0.253 0.252 0.28
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Targeting Autophagy for Cancer Treatment and Tumor Chemosensitization.
Pérez-Hernández et al. (2019)
13 more

5 mentions without citation

AMP-activated protein kinase selectively inhibited by the type II inhibitor SBI-0206965.
Dite et al. (2018)
4 more