› 6bz1

X-ray diffraction
2.97Å resolution

MEF2 Chimera D83V mutant/DNA complex

Released:
DOI: 10.2210/pdb6bz1/pdb
PDB entry 6bz1 coloured by chain, front view.
PDB entry 6bz1 coloured by chain, side view.
PDB entry 6bz1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Cancer Mutation D83V Induces an α-Helix to β-Strand Conformation Switch in MEF2B.
Lei X, Kou Y, Fu Y, Rajashekar N, Shi H, Wu F, Xu J, Luo Y, Chen L
J Mol Biol 430 1157-1172 (2018)
PMID: 29477338

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119640 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Myocyte-specific enhancer factor 2A; Myocyte-specific enhancer factor 2B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 95 amino acids
Theoretical weight: 11.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02080 (Residues: 1-8, 73-91; Coverage: 7%)
  • Canonical: Q02078 (Residues: 9-72, 92-95; Coverage: 13%)
Gene names: MEF2, MEF2A, MEF2B, XMEF2
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
DNA (5'-D(P*AP*AP*CP*TP*AP*TP*TP*TP*AP*TP*AP*AP*GP*A)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
DNA (5'-D(P*TP*TP*CP*TP*TP*AP*TP*AP*AP*AP*TP*AP*GP*TP*T)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 15 nucleotides
Theoretical weight: 4.57 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P32
Unit cell:
a: 78.44Å b: 78.44Å c: 111.041Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.141 0.139 0.19
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Functional and Regulatory Roles of Fold-Switching Proteins.
Kim et al. (2021)
4 more