› 6bzd

X-ray diffraction
2.67Å resolution

Structure of 14-3-3 gamma R57E mutant bound to GlcNAcylated peptide

Released:
DOI: 10.2210/pdb6bzd/pdb
PDB entry 6bzd coloured by chain, front view.
PDB entry 6bzd coloured by chain, side view.
PDB entry 6bzd coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins.
Toleman CA, Schumacher MA, Yu SH, Zeng W, Cox NJ, Smith TJ, Soderblom EJ, Wands AM, Kohler JJ, Boyce M
Proc Natl Acad Sci U S A 115 5956-5961 (2018)
PMID: 29784830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-158526 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein gamma Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 246 amino acids
Theoretical weight: 28.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61981 (Residues: 2-247; Coverage: 100%)
Gene name: YWHAG
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
GlcNAcylated peptide Chains: G, K
Molecule details ›
Chains: G, K
Length: 20 amino acids
Theoretical weight: 2.01 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 76.366Å b: 60Å c: 125.786Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.247 0.246 0.281
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Role of O-Linked N-Acetylglucosamine Protein Modification in Cellular (Patho)Physiology.
Chatham et al. (2021)
12 more

1 mention without citation

Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins.
Toleman et al. (2018)