› 6c2y

X-ray diffraction
2.74Å resolution

Human GRK2 in complex with Gbetagamma subunits and CCG257142

Released:
DOI: 10.2210/pdb6c2y/pdb
PDB entry 6c2y coloured by chain, front view.
PDB entry 6c2y coloured by chain, side view.
PDB entry 6c2y coloured by chain, top view.
Source organisms:
Primary publication:
Utilizing a structure-based docking approach to develop potent G protein-coupled receptor kinase (GRK) 2 and 5 inhibitors.
Waldschmidt HV, Bouley R, Kirchhoff PD, Lee P, Tesmer JJG, Larsen SD
Bioorg Med Chem Lett 28 1507-1515 (2018)
PMID: 29627263

Function and Biology Details

Reaction catalysed: 
ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150375 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-adrenergic receptor kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 689 amino acids
Theoretical weight: 79.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: P25098 (Residues: 1-689; Coverage: 100%)
Gene names: ADRBK1, BARK, BARK1, GRK2
Sequence domains:
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 77 amino acids
Theoretical weight: 8.67 KDa
Source organism: Bos taurus
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

1 bound ligand:
Ligand EJS 1 x EJS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 60.557Å b: 240.453Å c: 212.796Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.244 0.292
Expression system: Spodoptera aff. frugiperda 2 RZ-2014

Quick links

Citations

1 review citation

Therapeutic Targets for Treatment of Heart Failure: Focus on GRKs and β-Arrestins Affecting βAR Signaling.
Mangmool et al. (2018)
4 other articles

3 mentions without citation

Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1.
Chen et al. (2021)
2 more