› 6cbz

X-ray diffraction
1.65Å resolution

Estrogen Receptor Alpha Ligand Binding Domain Y537S Mutant in Complex with Estradiol and GRIP Peptide

Released:
DOI: 10.2210/pdb6cbz/pdb
PDB entry 6cbz coloured by chain, front view.
PDB entry 6cbz coloured by chain, side view.
PDB entry 6cbz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Endoxifen, 4-Hydroxytamoxifen and an Estrogenic Derivative Modulate Estrogen Receptor Complex Mediated Apoptosis in Breast Cancer.
Maximov PY, Abderrahman B, Fanning SW, Sengupta S, Fan P, Curpan RF, Rincon DMQ, Greenland JA, Rajan SS, Greene GL, Jordan VC
Mol Pharmacol 94 812-822 (2018)
PMID: 29739819

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136907 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Estrogen receptor Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P03372 (Residues: 305-554; Coverage: 42%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Estrogen receptor Chain: B
Molecule details ›
Chain: B
Length: 250 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P03372 (Residues: 305-554; Coverage: 42%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
grip peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 amino acids
Theoretical weight: 965 Da
Source organism: Homo sapiens
Expression system: synthetic construct

Ligands and Environments

2 bound ligands:
Ligand EST 2 x EST
Ligand GOL 1 x GOL
1 modified residue:
Ligand SCH 3 x SCH

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 56.126Å b: 84.621Å c: 58.587Å
α: 90° β: 108.48° γ: 90°
R-values:
R R work R free
0.184 0.184 0.214
Expression systems:
  • Escherichia coli
  • synthetic construct

Quick links

Citations

1 review citation

Next-Generation ERĪ± Inhibitors for Endocrine-Resistant ER+ Breast Cancer.
Fanning et al. (2019)
6 other articles

8 mentions without citation

Estrogen Receptor Complex to Trigger or Delay Estrogen-Induced Apoptosis in Long-Term Estrogen Deprived Breast Cancer.
Maximov et al. (2022)
7 more