› 6cds

X-ray diffraction
2.62Å resolution

Human neurofibromin 2/merlin/schwannomin residues 1-339 in complex with PIP2

Released:
DOI: 10.2210/pdb6cds/pdb
PDB entry 6cds coloured by chain, front view.
PDB entry 6cds coloured by chain, side view.
PDB entry 6cds coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Lipid binding promotes the open conformation and tumor-suppressive activity of neurofibromin 2.
Chinthalapudi K, Mandati V, Zheng J, Sharff AJ, Bricogne G, Griffin PR, Kissil J, Izard T
Nat Commun 9 1338 (2018)
PMID: 29626191

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Merlin Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 40.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35240 (Residues: 1-339; Coverage: 57%)
Gene names: NF2, SCH
Sequence domains:
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

4 bound ligands:
Ligand PIO 2 x PIO
Ligand PO4 2 x PO4
Ligand PEG 2 x PEG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 42.31Å b: 96.59Å c: 106.45Å
α: 90° β: 99.32° γ: 90°
R-values:
R R work R free
0.191 0.188 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Cytoskeletal Remodeling in Cancer.
Aseervatham J. (2020)
6 more

6 mentions without citation

Two Sides of the Coin: Ezrin/Radixin/Moesin and Merlin Control Membrane Structure and Contact Inhibition.
Michie et al. (2019)
5 more