6ces

Electron Microscopy
4Å resolution

Cryo-EM structure of GATOR1-RAG

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-130841 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
GATOR1 complex protein NPRL2 Chain: N
Molecule details ›
Chain: N
Length: 380 amino acids
Theoretical weight: 43.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8WTW4 (Residues: 1-380; Coverage: 100%)
Gene names: NPRL2, TUSC4
Sequence domains: Nitrogen permease regulator 2
GATOR1 complex protein NPRL3 Chain: M
Molecule details ›
Chain: M
Length: 569 amino acids
Theoretical weight: 63.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q12980 (Residues: 1-569; Coverage: 100%)
Gene names: C16orf35, CGTHBA, MARE, NPRL3
Sequence domains: Nitrogen Permease regulator of amino acid transport activity 3
GATOR1 complex protein DEPDC5 Chain: D
Molecule details ›
Chain: D
Length: 1603 amino acids
Theoretical weight: 181.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75140 (Residues: 1-1603; Coverage: 100%)
Gene names: DEPDC5, KIAA0645
Sequence domains:
Ras-related GTP-binding protein A Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 36.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7L523 (Residues: 1-313; Coverage: 100%)
Gene name: RRAGA
Sequence domains: Gtr1/RagA G protein conserved region
Structure domains:
Ras-related GTP-binding protein C Chain: C
Molecule details ›
Chain: C
Length: 399 amino acids
Theoretical weight: 44.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HB90 (Residues: 1-399; Coverage: 100%)
Gene name: RRAGC
Sequence domains: Gtr1/RagA G protein conserved region

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-7464
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)