› 6cfn

X-ray diffraction
2.5Å resolution

Crystal Structure of the DNA-free Glucocorticoid Receptor DNA Binding Domain

Released:
DOI: 10.2210/pdb6cfn/pdb
PDB entry 6cfn coloured by chain, front view.
PDB entry 6cfn coloured by chain, side view.
PDB entry 6cfn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The first crystal structure of a DNA-free nuclear receptor DNA binding domain sheds light on DNA-driven allostery in the glucocorticoid receptor.
Frank F, Okafor CD, Ortlund EA
Sci Rep 8 13497 (2018)
PMID: 30201977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucocorticoid receptor Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 90 amino acids
Theoretical weight: 10.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04150 (Residues: 418-506; Coverage: 12%)
Gene names: GRL, NR3C1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A

Ligands and Environments

1 bound ligand:
Ligand ZN 16 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 65.803Å b: 65.432Å c: 72.659Å
α: 71.27° β: 84.96° γ: 68.44°
R-values:
R R work R free
0.197 0.195 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

The Growth-Arrest-Specific (GAS)-5 Long Non-Coding RNA: A Fascinating lncRNA Widely Expressed in Cancers.
Goustin et al. (2019)
2 more

6 mentions without citation

The Non-Coding RNA GAS5 and Its Role in Tumor Therapy-Induced Resistance.
Lambrou et al. (2020)
5 more