› 6cnj

Electron Microscopy
3.4Å resolution

Structure of the 2alpha3beta stiochiometry of the human Alpha4Beta2 nicotinic receptor

Released:
DOI: 10.2210/pdb6cnj/pdb
PDB entry 6cnj coloured by chain, front view.
PDB entry 6cnj coloured by chain, side view.
PDB entry 6cnj coloured by chain, top view.
Source organisms:
Primary publication:
Structural principles of distinct assemblies of the human α4β2 nicotinic receptor.
Walsh RM, Roh SH, Gharpure A, Morales-Perez CL, Teng J, Hibbs RE
Nature 557 261-265 (2018)
PMID: 29720657
Related structures: EMD-7535

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210712 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Neuronal acetylcholine receptor subunit alpha-4 Chains: A, D
Molecule details ›
Chains: A, D
Length: 386 amino acids
Theoretical weight: 44.86 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P43681 (Residues: 27-364, 586-627; Coverage: 63%)
Gene names: CHRNA4, NACRA4
Sequence domains:
Neuronal acetylcholine receptor subunit beta-2 Chains: B, C, E
Molecule details ›
Chains: B, C, E
Length: 403 amino acids
Theoretical weight: 46.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P17787 (Residues: 26-353, 446-502; Coverage: 81%)
Gene name: CHRNB2
Sequence domains:
IgG1 Kappa Light Chain Chains: F, H, J
Molecule details ›
Chains: F, H, J
Length: 238 amino acids
Theoretical weight: 26.38 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
IgG1 Heavy Chain Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 462 amino acids
Theoretical weight: 51.2 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
4 bound ligands:
Ligand NAG 2 x NAG
Ligand NCT 2 x NCT
Ligand Y01 10 x Y01
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-7535
Expression system: Homo sapiens

Quick links

Citations

18 review citations

Nicotinic acetylcholine receptors: Conventional and unconventional ligands and signaling.
Papke et al. (2020)
17 more

26 mentions without citation

Progress in nicotinic receptor structural biology.
Gharpure et al. (2020)
25 more