Function and Biology Details
Reactions catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-[(E3-independent) ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
SdcA and Ubiquitin-conjugating enzyme E2 D3 (preferred)
PDBe Complex ID:
PDB-CPX-158194 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein SdcA
Molecule details ›
Chain: A
Length: 538 amino acids
Theoretical weight: 62.01 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
Sequence domains: SidC N-terminal domain
Length: 538 amino acids
Theoretical weight: 62.01 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
- Canonical:
Q6RCR3 (Residues: 1-538; Coverage: 59%)
Sequence domains: SidC N-terminal domain
Ubiquitin-conjugating enzyme E2 D3
Molecule details ›
Chain: B
Length: 149 amino acids
Theoretical weight: 16.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Ubiquitin-conjugating enzyme
Length: 149 amino acids
Theoretical weight: 16.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P61077 (Residues: 1-147; Coverage: 100%)
Sequence domains: Ubiquitin-conjugating enzyme
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
CHESS BEAMLINE A1
Spacegroup:
C2221
Expression system: Escherichia coli
