› 6crf

X-ray diffraction
2.62Å resolution

Crystal Structure of Shp2 E76K GOF Mutant in the Open Conformation

Released:
DOI: 10.2210/pdb6crf/pdb
PDB entry 6crf coloured by chain, front view.
PDB entry 6crf coloured by chain, side view.
PDB entry 6crf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural reorganization of SHP2 by oncogenic mutations and implications for oncoprotein resistance to allosteric inhibition.
LaRochelle JR, Fodor M, Vemulapalli V, Mohseni M, Wang P, Stams T, LaMarche MJ, Chopra R, Acker MG, Blacklow SC
Nat Commun 9 4508 (2018)
PMID: 30375388

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 526 amino acids
Theoretical weight: 60.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06124 (Residues: 1-525; Coverage: 89%)
Gene names: PTP2C, PTPN11, SHPTP2
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 249.104Å b: 41.735Å c: 153.853Å
α: 90° β: 125.76° γ: 90°
R-values:
R R work R free
0.2 0.198 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Turn and Face the Strange: A New View on Phosphatases.
Köhn M. (2020)
9 more

13 mentions without citation

Overcoming Immune Checkpoint Therapy Resistance with SHP2 Inhibition in Cancer and Immune Cells: A Review of the Literature and Novel Combinatorial Approaches.
Tojjari et al. (2023)
12 more